Antibacterial Activity of Leaf, Stem and Flower Extracts of Songgolangit Plant Against Staphylococcus aureus, Escherichia coli, and Pectobacterium sp.
DOI:
https://doi.org/10.54518/rh.6.4.2026.1505Keywords:
Antibacterial, Escherichia coli, Pectobacterium sp, Staphylococcus aureus, Tridax procumbensAbstract
The increasing prevalence of antibiotic-resistant bacteria has encouraged the exploration of natural sources of antibacterial compounds, including Tridax procumbens L. This study aimed to evaluate the antibacterial activity of leaf, stem, and flower extracts of T. procumbens against Staphylococcus aureus, Escherichia coli, and Pectobacterium sp. Plant samples were collected in Denpasar, Bali, dried, powdered, and extracted by maceration using 96% ethanol, n-hexane, and ethyl acetate. Antibacterial activity was assessed using the disc diffusion method at extract concentrations of 10–50%, with 1% DMSO as the negative control. Inhibition zone diameters were analyzed using analysis of variance followed by the least significant difference test at the 5% significance level. Leaf and stem extracts showed no antibacterial activity against any tested bacteria. Antibacterial activity was observed only in the flower extracts against S. aureus, with the n-hexane extract producing the largest inhibition zone (10.0 mm at 50% concentration), followed by the ethyl acetate extract, while the ethanol extract was inactive. No extracts inhibited E. coli or Pectobacterium sp. These findings indicate that n-hexane flower extract possesses limited antibacterial activity against S. aureus, highlighting its potential as a source of antibacterial compounds and the need for further purification and characterization of its bioactive constituents.
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Akinduti, P. A., Emoh-Robinson, V., Obamoh-Triumphant, H. F., Obafemi, Y. D., & Banjo, T. T. (2022). Antibacterial activities of plant leaf extracts against multi-antibiotic resistant Staphylococcus aureus associated with skin and soft tissue infections. BMC Complementary Medicine and Therapies, 22(1), 47-56. https://doi.org/10.1186/s12906-022-03527-y.
Al-Dhabi, N. A., Arasu, M. V., & Rejiniemon, T. S. (2015). In vitro antibacterial, antifungal, antibiofilm, antioxidant, and anticancer properties of isosteviol isolated from endangered medicinal plant Pittosporum tetraspermum. Evidence-Based Complementary and Alternative Medicine, 7(3), 56-76. https://doi.org/10.1155/2015/164261Digital.
Ameer, K., Shahbaz, H. M., & Kwon, J. H. (2017). Green extraction methods for polyphenols from plant matrices and their byproducts: A review. Comprehensive Reviews in Food Science and Food Safety, 16(2), 295-315. https://doi.org/10.1111/1541-4337.12253.
Amutha, R., Sudha, A., & Pandiselvi, P. (2019). Tridax procumbens (coat buttons)-A gift of nature: An overview. In Pharmacological benefits of natural products (pp. 193–212). Cham: Springer.
Andriana, Y., Xuan, T. D., Quy, T. N., Minh, T. N., Van, T. M., & Viet, T. D. (2019). Antihyperuricemia, antioxidant, and antibacterial activities of Tridax procumbens L. Foods, 8(1), 21-36. https://doi.org/10.3390/foods8010021.
Ashraf, M. V., Pant, S., Khan, M. H., Shah, A. A., Siddiqui, S., Jeridi, M., ... & Ahmad, S. (2023). Phytochemicals as antimicrobials: prospecting Himalayan medicinal plants as source of alternate medicine to combat antimicrobial resistance. Pharmaceuticals, 16(6), 881-892. https://doi.org/10.3390/ph16060881.
Atanasov, A. G., Zotchev, S. B., Dirsch, V. M., & Supuran, C. T. (2021). Natural products in drug discovery: advances and opportunities. Nature reviews Drug discovery, 20(3), 200-216. https://doi.org/10.1038/s41573-020-00114-z.
Bharathi, V., Varalakshmi, B., Gomathi, S., Shanmugapriya, A., & Karpagam, T. (2012). Antibacterial activity of Tridax procumbens Linn. International Journal of Pharmaceutical Sciences and Research, 3(4), 364–367.
Bouyahya, A., Chamkhi, I., Balahbib, A., Rebezov, M., Shariati, M. A., Wilairatana, P., ... & El Omari, N. (2022). Mechanisms, anti-quorum-sensing actions, and clinical trials of medicinal plant bioactive compounds against bacteria: A comprehensive review. Molecules, 27(5), 14-24. https://doi.org/10.3390/molecules27051606.
Charkowski, A. O. (2018). The changing face of bacterial soft-rot diseases. Annual Review of Phytopathology, 56(1), 269–288. https://doi.org/10.1146/annurev-phyto-080417-050112.
Egan, A. J., Errington, J., & Vollmer, W. (2020). Regulation of peptidoglycan synthesis and remodelling. Nature Reviews Microbiology, 18(8), 446-460. https://doi.org/10.1038/s41579-020-0366-3.
Farhan, M. S., Jaafar, F. R., & Oraibi, A. I. (2024). Unveiling the dual anti-viral and anti-bacterial potential of Tridax procumbens: integrating system biology and molecular modelling for therapeutic insights. Pharmacia, 71(9), 1-14. https://doi.org/10.3897/pharmacia.71.e134884.
Gbadamosi, A. S., Oseni, M. O., Adetukonbo, B. A., & Oseni, O. A. (2024). Biochemical characterization, antimicrobial potency, and metallic nanoparticle generation from tridax procumbens extracts. Asian Plant Research Journal, 12(4), 78-94. https://doi.org/10.9734/aprj/2024/v12i4264.
Gherardi, G. (2023). Staphylococcus aureus infection: Pathogenesis and antimicrobial resistance. International Journal of Molecular Sciences, 24(9), 81-94. https://doi.org/10.3390/ijms24098182.
Gopinathan, N., Balaji, B., Gokul Kumar, K., & Senthil Raj. (2024). Assessing the antibacterial activity of Tridax procumbens in ethanol extract. Virdi International Journal, 7(4), 66-88.
Ikala, R. O., Mshelia, M. B., Innocent, O. O., Gberikon, M. G., & Ebah, E. E. (2024). Phytochemical profile and in vitro antibacterial activity of Tridax procumbens leaf extract on multidrug-resistant E. coli and S. aureus clinical isolates. Medical and Health Sciences European Journal, 8(5), 33–45.
Khameneh, B., Iranshahy, M., Soheili, V., & Fazly Bazzaz, B. S. (2019). Review on plant antimicrobials: a mechanistic viewpoint. Antimicrobial Resistance & Infection Control, 8(1), 118-136. https://doi.org/10.1186/s13756-019-0559-6.
Kumar, O. A., & Naidu, L. M. (2011). Antibacterial potential of Tridax procumbens L. against human pathogens. Pharmasm, 2(2), 1-16.
Laoth, G. L., Wirya, G. N. A. S., & Temaja, I. G. R. M. (2026). In vitro evaluation of bacillus subtilis and pseudomonas fluorescens against klebsiella variicola in kepok banana. Research Horizon, 6(2), 861-872. https://doi.org/10.54518/rh.6.2.2026.1076.
Mundada, S., & Shivhare, R. (2010). Pharmacology of tridax procumbens: A review. International Journal of Pharmacology and Technology Research, 2(2), 1391–1394.
Murray, C. J., Ikuta, K. S., Sharara, F., Swetschinski, L., Aguilar, G. R., Gray, A., ... & Tasak, N. (2022). Global burden of bacterial antimicrobial resistance in 2019: A systematic analysis. The Lancet, 29(15), 629-655. https://doi.org/10.1016/S0140-6736(21)02724-0.
Nascimento, J. B., & da Costa, J. G. M. (2025). Flavonoids: a review of antibacterial activity against gram‐negative bacteria. International Journal of Microbiology, 20(1), 996-1021. https://doi.org/10.1155/ijm/9961121Digital.
Oulahal, N., & Degraeve, P. (2022). Phenolic-rich plant extracts with antimicrobial activity: an alternative to food preservatives and biocides?. Frontiers in Microbiology, 12(5), 753-768. https://doi.org/10.3389/fmicb.2022.753518.
Pan, H., Nie, S., Li, C., Zhang, L., Sun, X., Elateeq, A. A., ... & Fu, Y. (2023). A green and novel strategy based on CO2-responsive surfactant-functionalized multi-walled carbon nanotubes with microwave-ultrasound assistant to improve extraction and enrichment of phytochemicals from plant waste. Industrial Crops and Products, 19(5), 116-124. https://doi.org/10.1016/j.indcrop.2023.116294.
Rahman, Y., Suada, I. K., Wijaya, I. N., Wirawan, G. P., Phabiola, T. A., & Darmawati, I. A. P. (2024). Phytochemical and antioxidant activity of the ethanol extracts of Songgolangit plant (Tridax procumbens L.). Tropical Journal of Phytochemistry and Pharmaceutical Sciences, 3(5), 314–318. https://doi.org/10.26538/tjpps/v3i5.10.
Riu, F., Ruda, A., Ibba, R., Sestito, S., Lupinu, I., Piras, S., ... & Carta, A. (2022). Antibiotics and carbohydrate-containing drugs targeting bacterial cell envelopes: an overview. Pharmaceuticals, 15(8), 942-956. https://doi.org/10.3390/ph15080942.
Singh, N., Chhangani, N., & Bissa, S. (2025). Antibacterial potential and phytochemical analysis of two ethnomedicinally important plants. Current Research in Microbial Sciences, 8(4), 100-117. https://doi.org/10.1016/j.crmicr.2024.100297.
Syed, A., Benit, N., Alyousef, A. A., Alqasim, A., & Arshad, M. (2020). In-vitro antibacterial, antioxidant potentials and cytotoxic activity of the leaves of Tridax procumbens. Saudi Journal of Biological Sciences, 27(2), 757–761. https://doi.org/10.1016/j.sjbs.2019.12.024.
Yan, Y., Xia, X., Fatima, A., Zhang, L., Yuan, G., Lian, F., & Wang, Y. (2024). Antibacterial activity and mechanisms of plant flavonoids against gram-negative bacteria based on the antibacterial statistical model. Pharmaceuticals, 17(3), 292-304. https://doi.org/10.3390/ph17030292.
Yang, L., Wen, K. S., Ruan, X., Zhao, Y. X., Wei, F., & Wang, Q. (2018). Response of plant secondary metabolites to environmental factors. Molecules, 23(4), 762-776. https://doi.org/10.3390/molecules23040762.
Zhang, Z., Cao, M., Shang, Z., Xu, J., Chen, X., Zhu, Z., ... & Zhang, J. (2025). Research progress on the antibacterial activity of natural flavonoids. Antibiotics, 14(4), 334- 344. https://doi.org/10.3390/antibiotics14040334.
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Copyright (c) 2026 Yuliatin Rahman, I Ketut Suada, I Nyoman Wijaya, I Gede Putu Wirawan, Trisna Agung Phabiola, Khamdan Khalimi
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